Protein Name & Synonyms:
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~33 kDa band, corresponding to PPARg (isoform CRA-c) in samples from human and mouse origins. Reactivity to other species has not been tested.
PPARG, PPAR G, PPAR g, peroxisome proliferator activated receptor
PPAR (Peroxisome proliferator-activated receptor) is a member of the nuclear hormone receptor superfamily and functions as a transcriptional activator. PPARg is preferentially expressed in adipocyte as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipids metabolism, PPARg also modulates insulin sensitivity, cell proliferation and inflammation. The inhibition of PPARg transcription activity is due to MAP kinase phosphorylation at serine 84 of PPARg.
Synthetic peptide surrounding amino acid 262 of human PPARg isoform CRA-c
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
-20 °C. The shelf life is 12 months.