Rabbit anti-PPP1R14A Antibody

PPP1R14A Polyclonal Antibody

$320.00  
144-10160-100
+

Antigen Information

Accession Number:
  • Q96A00
Gene ID:
  • 94274
Gene Symbols:
  • PPP1R14A
Protein Name & Synonyms:
PPP1R14A, CPI-17, CPI17, PPP1INL, protein phosphatase 1 regulatory subunit 14A
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse, Rat
Clonality:
Polyclonal
Isotype:
IgG

Background

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
13kDa/16kDa
Observed MW
20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PPP1R14A antibody (144-10160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - PPP1R14A Polyclonal Antibody (144-10160)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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