Protein Name & Synonyms:
PPP6R2, KIAA0685, PP6R2, SAP190, SAPS2, protein phosphatase 6 regulatory subunit 2
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 640-959 of human PPP6R2 (NP_001229827.1).
Western blot analysis of extracts of various cell lines, using PPP6R2 antibody (144-08359) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.