Rabbit anti-PRKDC Antibody

PRKDC Polyclonal Antibody

144-07716-50
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Antigen Information

Accession Number:
  • P78527
Gene ID:
  • 5591
Gene Symbols:
  • PRKDC
Protein Name & Synonyms:
PRKDC, DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350, DNA-dependent protein kinase catalytic subunit
Target Species:
  • Human

Antibody Specifications

Applications:
WB, IHC, IF
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
465kDa/469kDa
Observed MW
469kDa

Immunogen

A synthetic peptide of human PRKDC

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PRKDC antibody (144-07716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.