Protein Name & Synonyms:
PTGS1, COX1, COX3, PCOX1, PES-1, PGG/HS, PGHS-1, PGHS1, PHS1, PTGHS, prostaglandin G/H synthase 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants.
WB 1:500 - 1:2000
IHC 1:100 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PTGS1 (NP_001258094.1).
Western blot analysis of extracts of various cell lines, using PTGS1 antibody (144-07341) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver cancer using PTGS1 antibody (144-07341) at dilution of 1:100 (40x lens).