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770-729-2992
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Product Specifications
Size:
50 µg
Protein Information
Protein Name & Synonyms:
RAD9
Target Species:
- Yeast
Antibody Specifications
Applications:
ELISA (dilution: 1/50, 1/250), Western Blotting
Concentration (lot specific):
IgG concentration 0.5mg/ml
Preparation:
Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Yeast
This product detects a band of approximately 60kDa.
Specificity
This product detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.
This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.
This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
Preservative:
0.09% Sodium Azide (NaN3)
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
