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Antigen Information
Protein Name & Synonyms:
CINC-1 / GRO alpha / CXCL1
Target Species:
- Rat
Antibody Specifications
Applications:
ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml after reconstitution.
Preparation:
Antiserum to CXCL1 was raised by repeated immunisation of rabbits with highly purified antigen.
Reconstitution:
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Rat
Specificity
This product recognizes rat CXCL1 also known as GRO1 and CINC-1, an 8kD cytokine which promotes neutrophil chemotaxis and degranulation and is expressed predominantly by monocytes after activation, but is also expressed by fibroblasts, endothelial cells, synovial cells and several tumour cell lines.
Storage/Stability
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Format Type:
Purified
Preparation
Antiserum to CXCL1 was raised by repeated immunisation of rabbits with highly purified antigen.
Immunogen:
Recombinant rat CXCL1
Preservative:
None present
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
