Protein Name & Synonyms:
Collagen I
Applications:
Immunohistology - Frozen, ELISA (dilution: 1/10, 1/60), Immunofluorescence, Immunohistology - Paraffin
Reconstitution:
Reconstitute with 500 ul of sterile DI water. Vial contains 0.05 mg of lyophilized antibody.
Immunohistology:
It has been reported that this antibody is suitable for use on paraffin embedded formalin fixed sections following treatment with 1 mg/ml pepsin in 0.5 M acetic acid for 2 hours at 37°C and multiple buffer washes.
This product is specific for both native and heat denatured rat Collagen type I.Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Reconstitution:
Reconstitute with 500 µl of sterile DI water. Vial contains 0.05 mg of lyophilized antibody.
Immunogen:
Type I Collagen from rat tail tendon
Preservative:
Contains mannitol, dextran & salts
Expiration:
12 months from the date of shipment when stored properly.