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770-729-2992
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Product Specifications
Size:
0.1 ml
Protein Information
Protein Name & Synonyms:
DARPP-32 (pThr34)
Target Species:
- Rat
Antibody Specifications
Applications:
Western Blotting
Preparation:
Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
Species Reactivity:
Rat, Human, mouse
This product detects a band of approximately 32kDa in rat caudate lysates.
Specificity
This product is specific for rat DARPP-32, when phosphorylated at threonine 34.
DARPP-32 (also known as protein phosphatase 1 regulatory subunit 1B PP1R1B) is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission.
DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .
DARPP-32 (also known as protein phosphatase 1 regulatory subunit 1B PP1R1B) is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission.
DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5
Format Type:
Purified
Preparation
Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.
Preservative:
| 0.09% | Sodium Azide |
| 0.1% | Bovine Serum Albumin |
| 50% | Glycerol |
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
