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770-729-2992
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Product Specifications
Size:
0.1 ml
Protein Information
Protein Name & Synonyms:
NMDAR, NR2B (pTyr1252)
Target Species:
- Rat
Antibody Specifications
Applications:
Western Blotting
Preparation:
Antisera to rat NMDAR NR2B subunit pTyr1252 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Rat
This product detects a band/s of approximately 180kDa in rat hippocampal cell lysates.
Specificity
This product specifically recognizes the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyrosine 1252.
Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.
The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.
Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.
The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
10 mM Hepes pH7.5
Format Type:
Purified
Preparation
Antisera to rat NMDAR NR2B subunit pTyr1252 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Phosphopeptide corresponding to smino acid residues surrounding the phosphorylated tyrosine 1252 of the NR2B subunit of rat NMDA receptor.
Preservative:
| 0.09% | Sodium Azide (NaN3) | |
| 50% | Glycerol | |
| 0.01% | Bovine Serum Albumin |
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
