Rabbit Anti-Rat NMDAR, NR2B (pTyr1472)

Rabbit Anti-Rat NMDAR, NR2B (pTyr1472)

DS-PB-01460
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Antigen Information

Protein Name & Synonyms:
NMDAR, NR2B (pTyr1472)
Target Species:
  • Rat

Antibody Specifications

Applications:
Western Blotting
Preparation:
Antisera to NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Rat, Human, bovien, canine, chicken, monkey, zebrafish, mouse and rat based on sequence similarity

This product detects a band of approximately 180 kDa in rat brain hippocampal lysates. This antibody also labels proteins of 65kDa and 115kDa.

Specificity

This product specifically recognizes the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor when phosphorylated at Tyr1472.

Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue Homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability.

The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease (Popke, E.J. 2003) and also epilepsy and ischemic neuronal cell death.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5.
Format Type:
Purified

Preparation

Antisera to NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the NMDA receptor, which includes phosphorylated Tyr1472.
Preservative:
0.09% Sodium Azide
0.01% Bovine Serum Albumin
50% Glycerol

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.