Protein Name & Synonyms:
RANTES / CCL5
Applications:
ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml after reconstitution
Preparation:
Antisera to rat RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
Reconstitution:
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
This product recognizes rat RANTES, also known as CCL5, a chemoattractant for blood monocytes, memory T-helper cells and eosinophils. RANTES causes the release of histamine from basophils and activates eosinophils.
This antibody has not been cross-adsorbed and may react with homologous peptides from related species.
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antisera to rat RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
Immunogen:
Escherichia coli -derived recombinant protein corresponding to rat RANTES.
Preservative:
None present.
Expiration:
12 months from the date of shipment when stored properly.