Rabbit Anti-Rat TNF-alpha [+Biotin]

Rabbit Anti-Rat TNF-alpha [+Biotin]

$570.00
DS-PB-01978
+

Antigen Information

Protein Name & Synonyms:
TNF-alpha / TNF
Target Species:
  • Rat

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration:
IgG concentration 0.1 mg/ml after reconstitution
Preparation:
Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Rat

Specificity

This product recognizes rat tumour necrosis factor alpha (TNF alpha), a 17kD cytokine, which is produced by many cell types, including macrophages, monocytes, neutrophils and a population of activated NK cells.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:
Recombinant rat TNF alpha.
Preservative:
None Present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.