The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human S100A9 (NP_002956.1).
Western blot analysis of extracts of various cell lines, using S100A9 antibody (144-09842) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using S100A9 antibody (144-09842) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using S100A9 antibody (144-09842) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using S100A9 antibody (144-09842) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.