Protein Name & Synonyms:
SEC23A, CLSD, protein transport protein Sec23A
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SEC23A (NP_006355.2).
Western blot analysis of extracts of various cell lines, using SEC23A antibody (144-012101) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.