SUMO4, IDDM5, SMT3H4, SUMO-4, dJ281H8.4, small ubiquitin-like modifier 4
Accession Number
Q6EEV6
Gene Symbols
SUMO4
Gene Id
387082
Recommended Applications
Western Blotting,IF
Purity
Affinity purification
Background
This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:200
Format
Calculated MW
10kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO4 (NP_001002255.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SUMO4 antibody (144-07517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 40s.
Immunofluorescence analysis of A549 cells using SUMO4 antibody (144-07517). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
