Rabbit anti-TAF5 Antibody

TAF5 Polyclonal Antibody

$200.00  
144-07221-50
+

Antigen Information

Accession Number:
  • Q15542
Gene ID:
  • 6877
Gene Symbols:
  • TAF5
Protein Name & Synonyms:
TAF5, TAF(II)100, TAF2D, TAFII-100, TAFII100, TATA-box binding protein associated factor 5
Target Species:
  • Human

Antibody Specifications

Applications:
WB, IF
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse, Rat
Clonality:
Polyclonal
Isotype:
IgG

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
80kDa/86kDa
Observed MW
100kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse brain, using TAF5 antibody (144-07221) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TAF5 Polyclonal Antibody (144-07221)

Immunofluorescence analysis of A-549 cells using TAF5 antibody (144-07221). Blue: DAPI for nuclear staining.
Immunofluorescence - TAF5 Polyclonal Antibody (144-07221)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.