Protein Name & Synonyms:
THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO, thrombopoietin
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1).
Western blot analysis of extracts of various cell lines, using THPO antibody (144-01825) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.