Co-transcription of this gene and the neighboring downstream gene (ubiquitin-conjugating enzyme E2 variant 1) generates a rare read-through transcript, which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. The protein encoded by this individual gene lacks a UEV1 domain but includes three transmembrane regions. Alternative splicing results in multiple transcript variants.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human TMEM189 (NP_954580.1).
Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (144-08380) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using TMEM189 antibody (144-08380) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody (144-08380) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.