Protein Name & Synonyms:
TMOD1, D9S57E, ETMOD, TMOD, tropomodulin-1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a member of the tropomodulin family. The encoded protein is an actin-capping protein that regulates tropomyosin by binding to its N-terminus, inhibiting depolymerization and elongation of the pointed end of actin filaments and thereby influencing the structure of the erythrocyte membrane skeleton. Multiple transcript variants encoding the same protein have been found for this gene.
WB 1:1000 - 1:4000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-359 of human TMOD1 (NP_003266.1).
Western blot analysis of extracts of various cell lines, using TMOD1 antibody (144-04160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.