Protein Name & Synonyms:
TRIM45, RNF99, tripartite motif containing 45
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human TRIM45 (NP_001139107.1).
Western blot analysis of extracts of various cell lines, using TRIM45 antibody (144-012739) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.