Protein Name & Synonyms:
TRIOBP, DFNB28, HRIHFB2122, TAP68, TARA, dJ37E16.4, TRIO and F-actin-binding protein
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene, however some transcripts may be subject to nonsense-mediated decay (NMD).
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 180-310 of human TRIOBP (NP_008963.3).
Western blot analysis of extracts of various cell lines, using TRIOBP antibody (144-04485) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.