TTLL12, dJ526I14.2, tubulin tyrosine ligase like 12
Accession Number
Q14166
Gene Symbols
TTLL12
Gene Id
23170
Recommended Applications
Western Blotting,IHC
Purity
Affinity purification
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
74kDa
Observed MW
83kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 485-644 of human TTLL12 (NP_055955.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TTLL12 antibody (144-08197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver injury using TTLL12 antibody (144-08197) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TTLL12 antibody (144-08197) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TTLL12 antibody (144-08197) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.