Rabbit Anti-YWHAB

Rabbit Anti-YWHAB

DS-PB-00005
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Antigen Information

Protein Name & Synonyms:
YWHAB
Target Species:
  • Sheep

Antibody Specifications

Applications:
ELISA (dilution: , 1/3000), Immunohistology - Paraffin, Western Blotting
Preparation:
Antisera to sheep 14-3-3 beta were raised by repeated immunisations of rabbit with highly purified antigen.
Species Reactivity:
Sheep, Bovine, Human, Mouse, Rabbit, Rat

This product detects a band of approximately 30kDa in HEK293 cell lysates.

Specificity

This product recognizes the acetylated N-terminal of 14-3-3 beta. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it❏'s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

14-3-3 beta plays a role in the pathogenesis of systemic lupus erythematosus and is involved in negative regulation of p90 ribosomal S6 kinase activity and tuberous sclerosis tumour suppressor proteins. It also binds IGF-1 receptor, RAF1 and CDC25 phosphatases.

This product may not react with recombinant proteins that are not N-acetylated.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Serum - liquid
Format Type:
Serum

Preparation

Antisera to sheep 14-3-3 beta were raised by repeated immunisations of rabbit with highly purified antigen.

Immunogen:
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 beta.

Peptide sequence: Ac.TMDKSELVC
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.