Rabbit Anti-YWHAQ

Rabbit Anti-YWHAQ

DS-PB-00013
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Antigen Information

Protein Name & Synonyms:
YWHAQ
Target Species:
  • Sheep

Antibody Specifications

Applications:
ELISA (dilution: , 1/3000), Immunohistology - Paraffin, Western Blotting
Preparation:
Antisera to sheep 14-3-3 tau/theta were raised by repeated immunisations of rabbit with highly purified antigen.
Species Reactivity:
Sheep, Bovine, Chicken, Human, Mouse, Rabbit, Rat

This product detects a band of approximately 30kDa in HEK293 cell lysates.

Specificity

This product recognizes 14-3-3 tau/theta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it❏'s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.

This product may not react with recombinant proteins that are not N-acetylated.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Serum - liquid
Format Type:
Serum

Preparation

Antisera to sheep 14-3-3 tau/theta were raised by repeated immunisations of rabbit with highly purified antigen.

Immunogen:
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 tau/theta.

Peptide sequence: Ac.MEKTELIQKAC
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.