The growth and widespread use of mouse monoclonal antibody technology has created a need for a rapid, simple, and accurate means of determining immunoglobulin class & sub-class. Detection of specific Igisotypes is also a powerful tool in the study of immunoglobulin deficiency disorders, allergies, autoimmune diseases, malignancies, gastrointestinal disorders or repeated bacterial infections.
RayBiotech now offers Ig Isotyping Arrays for Mouse and Rat species. The mouse array is a glass chip-based multiplex assay which detects the 8 mouse immunoglobulin sub-classes (IgG1, IgG2a, IgG2b, IgG3, IgA, IgD, IgE, and IgM) plus the two light chain types (Kappa and Lambda). The rat array detects 6 rat immunoglobulin sub-classes (IgA, IgM, IgG1, IgG2a, IgG2b, IgG2c) and the two lith chain types (Kappa and Lambda). The arrays use the sandwich ELISA-based format; however, unlike traditional ELISA which requires multiple steps and washes, the Rapid Isotyping Arrays feature a streamlined, single incubation procedure that requires only 60 minutes of processing time. Moreover, up to 16 samples can be assayed simultaneously on one glass chip.
First, samples are mixed with the Cy3 equivalent dye-labeled detection antibody, and then applied to the array slide. After a wash step, the slides are then dried and scanned with a compatible laser scanner.
Results can be evaluated by visual inspection (qualitative) or by densitometric data extraction (semi-quantitative).
Ig Isotype Array Testing Service is available. For more details, click here.
The analyzer software (AAM-ISO-G1-SW / AAR-ISO-G1-SW) is available for one-step data com putation. An Excel-based program designed specifically for this array, the software tool outputs normalized digital values as well as isotype names. Simply copy and paste the densitometry values into the appropriate cells and the tool does the rest.
The isotype-specific capture antibodies in the kit have been tested to react only with their own isotype; they exhibit no reactivity with other Ig isotypes up to 1 μg/ml. The accuracy of the array is confirmed by isotype-predetermined monoclonal antibody controls and third-party ELISA-determined hybridoma supernatant samples.
The limit of detection (LOD) for each Ig heavy or light chain has been determined with purified immunoglobulin antigens to be in the nanogram range (see tables below).