Protein Name & Synonyms:
Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG) (Perlecan) (PLC) [Cleaved into: Endorepellin, LG3 peptide]
- Bovine (cow)
- Non-human Primates
- Porcine (pig)
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
A murine EHS laminin preparation was used as the immunogen for the Perlecan antibody.
Protein G affinity chromatography
Human, Monkey, Cow, Pig, Mouse and Fish. Other species not tested.
Rat IgG2a, kappa
This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Store the Perlecan antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).