Rat Anti-Mouse CD11b

Rat Anti-Mouse CD11b, 0.1 mg

DS-MB-03718
+

Antigen Information

Target Species:
  • Mouse

Antibody Specifications

Concentration:
IgG concentration 1.0 mg/ml
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Species Reactivity:
Mouse

Specificity

Rat anti Mouse CD11b antibody recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Species Cross Reactivity

Human, Rabbit

Format

Format Type
Purified IgG - liquid
Buffer
Phosphate buffered saline
Preservative Stabilizers
0.09% Sodium Azide
Approximate Protein Concentration
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Immunogen

T cell enriched splenocytes from B10 mice

Applications

Application NameYesNoNot DeterminedSuggested Dilution
ELISA  X 
Flow CytometryX  1/50 - 1/200
ImmunofluorescenceX   
Immunohistology - FrozenX   
Immunohistology - Paraffin*X   
Immunohistology - Resin  X 
ImmunoprecipitationX   
Western Blotting  X 

Storage/Stability

Storage
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of dispatch.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.