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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Target Species:
- Mouse
Antibody Specifications
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Species Reactivity:
Mouse
Specificity
Rat anti Mouse CD11b antibody recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Species Cross Reactivity
Human, Rabbit
Format
Format Type
Purified IgG - liquid
Buffer
Phosphate buffered saline
Preservative Stabilizers
0.09% Sodium Azide
Approximate Protein Concentration
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Immunogen
T cell enriched splenocytes from B10 mice
Applications
| Application Name | Yes | No | Not Determined | Suggested Dilution |
| ELISA | X | |||
| Flow Cytometry | X | 1/50 - 1/200 | ||
| Immunofluorescence | X | |||
| Immunohistology - Frozen | X | |||
| Immunohistology - Paraffin* | X | |||
| Immunohistology - Resin | X | |||
| Immunoprecipitation | X | |||
| Western Blotting | X |
Storage/Stability
Storage
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of dispatch.
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
