Protein Name & Synonyms:
Immunohistology - Frozen, Flow Cytometry, Immunoprecipitation, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
This product recognizes a 30kD glycoprotein in immunoprecipitation and Western blotting assays.
This product recognizes the murine Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody.
This antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Store at +4 °C for one month or at -20 °C for longer.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
12 months from the date of shipment when stored properly.