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770-729-2992
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Product Specifications
Size:
100 TESTS
Protein Information
Protein Name & Synonyms:
F4/80 Ag.
Target Species:
- Mouse
Antibody Specifications
Applications:
Flow Cytometry
Reconstitution:
Reconstitute with 1 ml distilled water
Species Reactivity:
Mouse
Specificity
The F4/80 antibody recognizes the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node.
The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node.
The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Storage/Stability
Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1 ml distilled water
Preparation
Immunogen:
Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice
Preservative:
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
| 5% | Sucrose |
This product
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
