Rat Anti-Mouse F4/80 Ag. [+RPE]

Rat Anti-Mouse F4/80 Ag. [+RPE]

$548.00
DS-MB-03911
+

Antigen Information

Protein Name & Synonyms:
F4/80 Ag.
Target Species:
  • Mouse

Antibody Specifications

Applications:
Flow Cytometry, Immunofluorescence
Reconstitution:
Reconstitute with 1 ml distilled water
Species Reactivity:
Mouse

Specificity

The F4/80 antibody recognizes the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node.

The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1 ml distilled water

Preparation

Immunogen:
Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice
Preservative:
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
This product
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.