Rat Anti-Mouse GITRL

Rat Anti-Mouse GITRL
CODE:  DS-MB-01367

Antigen Information

Protein Name & Synonyms:
GITRL
Target Species:
  • Mouse

Antibody Specifications

Applications:
ELISA (dilution: 1/25 , 1/50), Flow Cytometry
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Mouse

Specificity

This product recognizes mouse glucocorticoid-induced tumour necrosis factor receptor ligand (GITRL), a 20 kD member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18.

Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation.

Studies demonstrate that GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naïve and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Recombinant mouse GITRL
Preservative:
0.09%Sodium Azide
This product
Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 myeloma cell line.