ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 2-5 µg/mL)
Immunogen was whole expressing mouse IL-4. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized rat. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography.
Supplied as 100 µl of purified antibody in sterile PBS (pH 7.5), containing 0.1% BSA. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles.
IL-4 is a T cell product originally described as B cell growth factor by virtue of its ability to co-stimulate B lymphocyte proliferation (1). It has broad effects on B cells, including the promotion of Ig class switching to IgE and IgGl isotypes in activated B cells. In addition to regulating B lymphocyte growth and differentiation, IL-4 modulates the survival, proliferation, and differentiation of T lymphocytes, as well as a wide range of hematopoietic cell lineages, thereby contributing to allergic and other immune processes.
The antibody specifically detected protein derived from rat. It also recognizes tested samples containing IL-4 in both ELISA and WB. This antibody shows no cross-reactivity with human IL-4 and other unrelated proteins.
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
- Lebman, D. A., A. Lebman, and R. L. Coffman. 1988. Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures.J. Exp. Med. 168, 853 .
- Kepron, M. R., Y.W. Chen, J. W. Uhr, and E. S. Vitetta. 1989. IL-4 induces the specific rearrangement of yl genes on the expressed and unexpressed chromosomes of lipopolysaccharide-activated normal murine cells. f. Immunol. 143:334.