Protein Name & Synonyms:
TNF-alpha / TNF
Applications:
Immunohistology - Frozen, ELISA (dilution: 1/50 , 1/100), Flow Cytometry, Functional Assays
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Mouse, Rat
This product is specific for mouse tumour necrosis factor alpha (TNF alpha), a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.
This product is reported to neutralise the effects of mouse TNF alpha (4,5).
Store at -20 °C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Quality Control (Contaminants):
<0.01 EU/ug
Format Type:
Low Endotoxin
Immunogen:
Recombinant murine TNF alpha expressed in E. coli
Preservative:
None present