Protein Name & Synonyms:
Immunohistology - Frozen, ELISA (dilution: 1/100, 1/1000), Immunofluorescence, Immunoprecipitation, Radioimmunoassays, Western Blotting
IgG concentration 1.0 mg/ml
Yeast, birds and mammals
This product is suitable for use as a loading control.
This product recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin), see Wehland, J. et al. for details. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin, see Burns, R. for details.
This product is routinely tested in ELISA on Tubulin.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.