Rat Anti-Tubulin alpha

Rat Anti-Tubulin alpha

DS-MB-02478
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Antigen Information

Protein Name & Synonyms:
Tubulin alpha
Target Species:
  • Yeast

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: 1/100, 1/1000), Immunofluorescence, Immunoprecipitation, Radioimmunoassays, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Species Reactivity:
Yeast, birds and mammals

This product is suitable for use as a loading control.

Specificity

This product recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin), see Wehland, J. et al. for details. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin, see Burns, R. for details.

This product is routinely tested in ELISA on Tubulin.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Immunogen:
Yeast tubulin
Preservative:
0.09%Sodium Azide
This product
Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.