Rat Anti-Tubulin alpha

Rat Anti-Tubulin alpha


Product Specifications

0.5 mg

Protein Information

Protein Name & Synonyms:
Tubulin alpha
Target Species:
  • Yeast

Antibody Specifications

Immunohistology - Frozen, ELISA (dilution: 1/100, 1/1000), Immunofluorescence, Immunoprecipitation, Radioimmunoassays, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Species Reactivity:
Yeast, birds and mammals

This product is suitable for use as a loading control.


This product recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin), see Wehland, J. et al. for details. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin, see Burns, R. for details.

This product is routinely tested in ELISA on Tubulin.


Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.


Supplied as:
Purified IgG - liquid
Phosphate buffered saline
Format Type:


Yeast tubulin
0.09%Sodium Azide
This product
Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
12 months from the date of shipment when stored properly.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.