Rat Cytokine Array C1

RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.

$411.47  
AAR-CYT-1-4
+

Antigen Information

Gene Symbols:
CCL2 | CCL20 | CNTF | CSF2 | CX3CL1 | CXCL2 | CXCL3 | CXCL5 | IFNG | IL10   View more

Assay Format

Number of Targets Detected:
19
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-4
IL-6
IL-10
LIX
Leptin
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
beta-NGF
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries.?Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample type: Conditioned Media
  2. Zhou J, Li J, Rosenbaum DM, Barone FC. Thrombopoietin protects the brain and improves sensorimotor functions: reduction of stroke-induced MMP-9 upregulation and blood-brain barrier injury.?Journal of Cerebral Blood Flow & Metabolism. 2011;31(3):924-933. doi:10.1038/jcbfm.2010.171.
    Species: Rat
    Sample type: Tissue lysate
  3. Zhang H, Yang R, Wang Z, Lin G, Lue TF, Lin C-S. Adipose Tissue-Derived Stem Cells Secrete CXCL5 Cytokine with Neurotrophic Effects on Cavernous Nerve Regeneration.?The journal of sexual medicine. 2011;8(2):437-446. doi:10.1111/j.1743-6109.2010.02128.x.
    Species: Rat
    Sample type: Conditioned Media
  4. Jung, Eun-Jung, et al. "Bone marrow-derived mesenchymal stromal cells support rat pancreatic islet survival and insulin secretory function in vitro."?Cytotherapy?13.1 (2011): 19-29.
    Species: Rat
    Sample type: Conditioned Media
  5. Cinova, Jana, et al. "Role of intestinal bacteria in gliadin-induced changes in intestinal mucosa: study in germ-free rats."?PloS one?6.1 (2011): e16169.
    Species: Rat
    Sample type: Conditioned Media
  6. Yang, Zhikai, et al. "Low-level laser irradiation alters cardiac cytokine expression following acute myocardial infarction: a potential mechanism for laser therapy."?Photomedicine and laser surgery?29.6 (2011): 391-398.
    Species: Rat
    Sample type: Tissue lysate
  7. Kudo, Tomohiro, et al. "Topical application of glycyrrhizin preparation ameliorates experimentally induced colitis in rats."?World J Gastroenterol?17.17 (2011): 2223-2228.
    Species: Rat
    Sample type: Tissue Lysate
  8. Calvillo, Laura, et al. "Vagal stimulation, through its nicotinic action, limits infarct size and the inflammatory response to myocardial ischemia and reperfusion."?Journal of cardiovascular pharmacology?58.5 (2011): 500-507.
    Species: Rat
    Sample type: Plasma
  9. Yadav, Umesh CS, et al. "Amelioration of experimental autoimmune uveoretinitis by aldose reductase inhibition in Lewis rats."?Investigative ophthalmology & visual science?52.11 (2011): 8033-8041.
    Species: Rat
    Sample type: aqueous humor
  10. Obinata, Daisuke, et al. "Transplantation of mature adipocyte?derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model." International Journal of Urology 18.12 (2011): 827-834.
    Species: Rat
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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