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Rat Cytokine Array C1

RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.

$646.60  
AAR-CYT-1-8
+

Antigen Information

Gene Symbols:
CCL2 | CCL20 | CNTF | CSF2 | CX3CL1 | CXCL2 | CXCL3 | CXCL5 | IFNG | IL10   View more

Assay Format

Number of Targets Detected:
19
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-4
IL-6
IL-10
LIX
Leptin
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
beta-NGF
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Senoo, Kyoko, et al. "Expression of optineurin isolated from rat-injured dental pulp and the effects on inflammatory signals in normal rat kidney cells."?Odontology?106.2 (2018): 135-144.
    Species: Rat
    Sample type: Cell Culture Supernatant
  2. Shi S., Liang D., Bao M., et al. Gx-50 Inhibits Neuroinflammation via ?7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways. J Alzheimers Dis. 2016 Jan 21. Epub ahead of print
    Species: Mouse
    Sample type: Conditioned Media
  3. Tsai T., Lee T., Liu M., et al. Release of endogenous heat shock protein 72 on the survival of sepsis in rats. J Surg Res. 2015 Sep;198(1):165-74. doi: 10.1016/j.jss.2015.05.004.
    Species: Rat
    Sample type: Serum
  4. Sala R., Mena P., Savi M., et al. Urolithins at physiological concentrations affect the levels of pro-inflammatory cytokines and growth factor in cultured cardiac cells in hyperglucidic conditions. Journal of Functional Foods Volume 15, May 2015, Pages 97-105. doi:10.1016/j.jff.2015.03.019
    Species: Rat
    Sample type: Conditioned Media
  5. Gu P., Qiu F., Han R., et al. Efficient differentiation of neural stem cells induced by the rat bone marrow stromal cells. Int J Clin Exp Med. 2015 May 15;8(5):6713-24.
    Species: Rat
    Sample type: Conditioned Media
  6. Deuse T., Hua X., Wang D., Maegdefessel L., et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature (2014) doi:10.1038/nature13232 [Epub ahead of print]
    Species: Rat
    Sample type: Tissue Lysate
  7. Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014
    Species: Rat
    Sample type: Tissue Lysate
  8. Kinoshita, Manao, et al. "Secretion of matrix metalloproteinase-9 from astrocytes by inhibition of tonic P2Y14-receptor-mediated signal (s)."?Cellular and molecular neurobiology?33.1 (2013): 47-58.
    Species: Rat
    Sample type: Conditioned Media
  9. Ning, Hongxiu, et al. "Effects of EdU labeling on mesenchymal stem cells."?Cytotherapy?15.1 (2013): 57-63.
    Species: Rat
    Sample type: Conditioned Media
  10. Gruber-Schoffnegger, D., Drdla-Schutting, R., HÃ?nigsperger, C., Wunderbaldinger, G., Gassner, M., & SandkÃ?hler, J. (2013). Induction of thermal hyperalgesia and synaptic long-term potentiation in the spinal cord lamina I by TNF-? and IL-1? is mediated by glial cells.?Journal of Neuroscience,?33(15), 6540-6551.
    Species: Rat
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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