Rat Cytokine Array C1

RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.

$647.00  
AAR-CYT-1-8
+

Assay Format

Number of Targets Detected:
19
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
CCL2 | CCL20 | CNTF | CSF2 | CX3CL1 | CXCL2 | CXCL3 | CXCL5 | IFNG | IL10   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-4
IL-6
IL-10
LIX
Leptin
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
beta-NGF
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Deuse T., Hua X., Wang D., Maegdefessel L., et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature (2014) doi:10.1038/nature13232
    Species: Rat
    Sample type: Tissue Lysate (Rat Injured Aortas)
  2. Schmelzer K, Wheelock Å, Dettmer K , Morin D, Hammock B.The role of Inflammatory Mediators in the Synergistic Toxicity of Ozone and 1-Nitronaphthalene in Rat Airways.Environ Health Perspect. 2006 ; 114(9): 1354-1360.
    Species: Rat
    Sample type: BAL Fluid (bronchioalveolar lavage fluid (BALF))
  3. Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014
    Species: Rat
    Sample type: Tissue Lysate (Rat Brain Tissue)
  4. Gruber-Schoffnegger, D., Drdla-Schutting, R., Hönigsperger, C., Wunderbaldinger, G., Gassner, M., & Sandkühler, J. (2013). Induction of thermal hyperalgesia and synaptic long-term potentiation in the spinal cord lamina I by TNF-? and IL-1? is mediated by glial cells. Journal of Neuroscience, 33(15), 6540-6551.
    Species: Rat
    Sample type: Conditioned Media (Spinal cord dorsal horn slices )
  5. Liu J., Xu C., Chen L., et al. Involvement of Kv1.3 and p38 MAPK signaling in HIV-1 glycoprotein 120-induced microglia neurotoxicity. Cell Death Dis. 2012 Jan 19;3:e254. doi: 10.1038/cddis.2011.140.
    Species: Rat
    Sample type: Conditioned Media (Microglial cell supernate after treatment with GP120)
  6. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample type: Conditioned Media (A7r5 cells )
  7. Zhou J, Li J, Rosenbaum DM, Barone FC. Thrombopoietin protects the brain and improves sensorimotor functions: reduction of stroke-induced MMP-9 upregulation and blood-brain barrier injury. Journal of Cerebral Blood Flow & Metabolism. 2011;31(3):924-933. doi:10.1038/jcbfm.2010.171.
    Species: Rat
    Sample type: Tissue lysate (cortical brain sample)
  8. Moreau, R et al. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Biochem. Pharmacol. Volume 69, Issue 1, Pages 29-40, Jan 2005
    Species: Rat
    Sample type: Serum
  9. Obinata, Daisuke, et al. "Transplantation of mature adipocyte?derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model." International Journal of Urology 18.12 (2011): 827-834.
    Species: Rat
    Sample type: Conditioned Media (DFAT cells)
  10. Sala R., Mena P., Savi M., et al. Urolithins at physiological concentrations affect the levels of pro-inflammatory cytokines and growth factor in cultured cardiac cells in hyperglucidic conditions. Journal of Functional Foods Volume 15, May 2015, Pages 97-105. doi:10.1016/j.jff.2015.03.019
    Species: Rat
    Sample type: Conditioned Media (Cardiomyocytes in culture)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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