Rat Cytokine Array C2

RayBio® C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.

$501.97  
AAR-CYT-2-4
+

Antigen Information

Gene Symbols:
AGER | AGRN | CCL2 | CCL20 | CD86 | CNTF | CSF2 | CX3CL1 | CXCL1 | CXCL2   View more

Assay Format

Number of Targets Detected:
34
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
Agrin
CD86
(B7-2)
beta-NGF
CINC-1
(CXCL1)
CINC-2
CINC-3
CNTF
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GM-CSF
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R6
(IL-1 Rrp2)
IL-2
IL-4
IL-6
IL-10
IL-13
Leptin
LIX
L-Selectin
(CD62L)
MCP-1
(CCL2)
MIP-3 alpha
(CCL20)
MMP-8
PDGF-AA
Prolactin R
RAGE
TCK-1
(CXCL7)
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Kitic, Maja, et al. "Intrastriatal injection of interleukin-1 beta triggers the formation of neuromyelitis optica-like lesions in NMO-IgG seropositive rats."?Acta neuropathologica communications?1.1 (2013): 5.
    Species: Rat
    Sample type: Cell Lysate
  2. De Astis, Silvia, et al. "Nanostructured TiO 2 surfaces promote polarized activation of microglia, but not astrocytes, toward a proinflammatory profile."?Nanoscale?5.22 (2013): 10963-10974.
    Species: Rat
    Sample type: Cell Lysate
  3. Delucchi F, Berni R, Frati C, et al. Resveratrol Treatment Reduces Cardiac Progenitor Cell Dysfunction and Prevents Morpho-Functional Ventricular Remodeling in Type-1 Diabetic Rats. Pietropaolo M, ed. PLoS ONE 2012;7(6):e39836. doi:10.1371/journal.pone.0039836.
    Species: Rat
    Sample type: Conditioned Media
  4. Campbell LR, Pang Y, et al: Intracerebral lipopolysaccharide induces neuroinflammatory change and augmented brain injury in growth-restricted neonatal rats. Pediatr Res. 2012 Jun;71(6):645-52. doi: 10.1038/pr.2012.26.
    Species: Rat
    Sample type: Tissue Lysate
  5. Grothusen C., Hagemann A., Attmann T., et al. Impact of an Interleukin-1 Receptor Antagonist and Erythropoietin on ExperimentalMyocardial Ischemia/Reperfusion Injury. The ScientificWorld Journal Volume 2012, Article ID 737585, 6 pages doi:10.1100/2012/737585
    Species: Rat
    Sample type: Serum
  6. Pillon N., Arane K., Bilan P., et al. Muscle cells challenged with saturated fatty acids mount an autonomous inflammatory response that activates macrophages. Cell Commun Signal. 2012 Oct 19;10(1):30. doi: 10.1186/1478-811X-10-30.
    Species: Rat
    Sample type: Conditioned Media
  7. Zhang, Ziyang, et al. "The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration."?Biomaterials?32.17 (2011): 4109-4117.
    Species: Rat
    Sample type: Conditioned Media
  8. Gillum, Matthew P., et al. "SirT1 regulates adipose tissue inflammation."?Diabetes?60.12 (2011): 3235-3245.
    Species: Rat
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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