Rat Cytokine Array C2

RayBio® C-Series Rat Cytokine Antibody Array 2 Kit. Detects 34 Rat Cytokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
AGER | AGRN | CCL2 | CCL20 | CD86 | CNTF | CSF2 | CX3CL1 | CXCL1 | CXCL2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
Fas Ligand
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R6
(IL-1 Rrp2)
MIP-3 alpha
Prolactin R
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. An S., Zong G., Wang Z., et al. Activation of protease-activated receptor 4 of mast cells could downregulate proinflammatory cytokines in irritable bowel syndrome. Gut . 2017 Nov;66(11):2040-2042. doi: 10.1136/gutjnl-2016-313504.
    Species: Rat
    Sample type: Conditioned Media (PAR4-AP treated Bone marrow mast cells)
  2. de Couto G., Liu W., Tseliou E., et al. Macrophages mediate cardioprotective cellular postconditioning in acute myocardial infarction. J Clin Invest. 2015 Aug 3;125(8):3147-62. doi: 10.1172/JCI81321.
    Species: Rat
    Sample type: Tissue Lysate (Heart lysate treated with cardiosphere derived cells)
  3. Zhang, Ziyang, et al. "The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration." Biomaterials 32.17 (2011): 4109-4117.
    Species: Rat
    Sample type: Conditioned Media
  4. Gillum, Matthew P., et al. "SirT1 regulates adipose tissue inflammation." Diabetes 60.12 (2011): 3235-3245.
    Species: Rat
    Sample type: Plasma (normal chow–fed control ASO– and SirT1 ASO–treated rats)
  5. Cho, Yoon, et al. "Identification of novel cytokine biomarkers of hexanal exposure associated with pulmonary toxicity." Environmental Pollution 229 (2017): 810-817.
    Species: Rat
    Sample type: Tissue Lysate (lung tissue)
  6. De Astis, Silvia, et al. "Nanostructured TiO 2 surfaces promote polarized activation of microglia, but not astrocytes, toward a proinflammatory profile." Nanoscale 5.22 (2013): 10963-10974.
    Species: Rat
    Sample type: Cell Lysate (microglial and astrocytes)
  7. Kitic, Maja, et al. "Intrastriatal injection of interleukin-1 beta triggers the formation of neuromyelitis optica-like lesions in NMO-IgG seropositive rats." Acta neuropathologica communications 1.1 (2013): 5.
    Species: Rat
    Sample type: Cell Lysate (primary rat brain endothelial cell)
  8. Meireles M., Marques C., Norberto S., et al. The impact of chronic blackberry intake on the neuroinflammatory status of rats fed a standard or high-fat diet. J Nutr Biochem. 2015 Nov;26(11):1166-73. doi: 10.1016/j.jnutbio.2015.05.008.
    Species: Rat
    Sample type: Tissue Lysate (Cortex brain tissue lysate)
  9. Attia, Mohamed, et al. "Early events of overused supraspinatus tendons involve matrix metalloproteinases and EMMPRIN/CD147 in the absence of inflammation." The American journal of sports medicine 41.4 (2013): 908-917.
    Species: Rat
    Sample type: Tissue Lysate (supraspinatus tendons)
  10. Perez-Ramirez I., Enciso-Moreno J., Guevara-Gonzalez R., et al. Modulation of renal dysfunction by Smilax cordifolia and Eryngium carlinae, and their effect on kidney proteome in obese rats. Journal of Functional Foods Volume 20, January 2016, Pages 545-555
    Species: Rat
    Sample type: Tissue lysate (Renal corticles from obese rats)

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