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Rat Cytokine Array Q3

Quantibody® Rat Cytokine Array 3 Kit. Detects 27 Rat Cytokines. Suitable for all liquid sample types.

Old price: $1,012.02
$708.41  
You save: $303.61 (30%)
QAR-CYT-3-1
+

Antigen Information

Gene Symbols:
AGER | CCL2 | CD86 | CNTF | CSF2 | CX3CL1 | CXCL1 | CXCL2 | CXCL3 | CXCL5   View more

Assay Format

Number of Targets Detected:
27
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
beta-NGF
CD86
(B7-2)
CINC-1
(CXCL1)
CINC-2
CINC-3
CNTF
Fractalkine
(CX3CL1)
GM-CSF
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-10
IL-13
IL-2
IL-4
IL-6
LIX
L-Selectin
(CD62L)
MCP-1
(CCL2)
PDGF-AA
Prolactin R
RAGE
TCK-1
(CXCL7)
TIMP-1
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Rat Cytokine Array Q3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Fischer G., Wang F., Xiang H., et al. Inhibition of neuropathic hyperalgesia by intrathecal bone marrow stromal cells is associated with alteration of multiple soluble factors in cerebrospinal fluid. Exp Brain Res. 2017 Sep;235(9):2627-2638. doi: 10.1007/s00221-017-5000-x.
    Species: Rat
    Sample type: CSF
  2. He J., Zhao J., Peng X., et al. Molecular Mechanism of MiR-136- 5p Targeting NF-?B/A20 in the IL-17- Mediated Inflammatory Response after Spinal Cord Injury. Cell Physiol Biochem. 2017;44(3):1224-1241. doi: 10.1159/000485452
    Species: Rat
    Sample type: Conditioned Media
  3. Hbiwer L., Dsouza K., Abidali A., et al. Time course of cardiac inflammation during nitric oxide synthase inhibition in SHR: impact of prior transient ACE inhibition. Hypertension Research 39, 8-18 (January 2016) | doi:10.1038/hr.2015.107
    Species: Rat
    Sample type: Tissue Lysate
  4. Xu D., Xiong H., Xiao Z., et al. Uterine cytokine profile in a rat model of endometritis. Am J Reprod Immunol. 2015 Mar;73(3):214-20. doi: 10.1111/aji.12330.
    Species: Rat
    Sample type: Tissue Lysate
  5. Levine YA, Koopman FA, Faltys M, Caravaca A, Bendele A, et al. (2014) Neurostimulation of the Cholinergic Anti-Inflammatory Pathway Ameliorates Disease in Rat Collagen-Induced Arthritis. PLoS ONE 9(8): e104530. doi:10.1371/journal.pone.0104530
    Species: Rat
    Sample type: Serum
  6. Knaub, Leslie A., et al. "Impaired response to exercise intervention in the vasculature in metabolic syndrome."?Diabetes and Vascular Disease Research?10.3 (2013): 222-238.
    Species: Rat
    Sample type: Serum
  7. Harati R, VillÃ?gier A-S, Banks WA, Mabondzo A. Susceptibility of juvenile and adult blood-brain barrier to endothelin-1: regulation of P-glycoprotein and breast cancer resistance protein expression and transport activity. Journal of Neuroinflammation 2012;9:273. doi:10.1186/1742-2094-9-273.
    Species: Rat
    Sample type: Tissue Lysate
  8. Harati R, Villégier A-S, Banks WA, Mabondzo A. Susceptibility of juvenile and adult blood-brain barrier to endothelin-1: regulation of P-glycoprotein and breast cancer resistance protein expression and transport activity. Journal of Neuroinflammation 2012;9:273. doi:10.1186/1742-2094-9-273.
    Species: Rat
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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