Rat Cytokine Array Q4

Quantibody® Rat Cytokine Array 4 Kit. Detects 40 Rat Cytokines. Suitable for all liquid sample types.

$1,285.73
QAR-CYT-4-1
+

Antigen Information

Gene Symbols:
ADIPOQ | CCL11 | CCL27 | CCL3 | CCL5 | CD48 | CD80 | CDH3 | DCN | EPHA5   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
  • Gene Symbol: TNFRSF9
  • Accession #: Q4W8J3
Activin A
Adiponectin
(ACRP30)
  • Gene Symbol: ADIPOQ
  • Gene ID: 246253
  • Accession #: Q8K3R4
CD80
(B7-1)
  • Gene Symbol: CD80
  • Accession #: Q62680
CD48
(SLAMF2)
CTACK
(CCL27)
  • Gene Symbol: CCL27
  • Gene ID: 362505
  • Accession #: D4AAL6
Decorin
Eotaxin-1
(CCL11)
EphA5
  • Gene Symbol: EphA5
  • Gene ID: 79208
  • Accession #: P54757
Erythropoietin
  • Gene Symbol: EPO
  • Gene ID: 24335
  • Accession #: P29676
FGF-BP
  • Gene Symbol: FGFBP1
  • Gene ID: 64535
  • Accession #: Q9QY10
Flt-3 Ligand
Galectin-1
Galectin-3
Gas 1
GFR alpha-1
(GDNF R)
  • Gene Symbol: GFRA1
  • Gene ID: 25454
  • Accession #: Q62997
gp130
  • Gene Symbol: Il6st
  • Accession #: P40190
HGF
IL-1 R6
(IL-1 Rrp2)
IL-1 ra
(IL-1 F3)
IL-2 R alpha
  • Gene Symbol: IL2RA
  • Gene ID: 25704
  • Accession #: P26897
IL-3
  • Gene Symbol: IL3
  • Accession #: P04823
IL-7
  • Gene Symbol: IL7
  • Accession #: P56478
IL-17F
  • Gene Symbol: IL17F
  • Gene ID: 301291
  • Accession #: Q5BJ95
IL-22
  • Gene Symbol: IL22
  • Gene ID: 500836
  • Accession #: G3V6X6
JAM-A
(CD321/F11R)
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 25542
  • Accession #: P50229
Neuropilin-1
  • Gene Symbol: NRP1
  • Gene ID: 246331
  • Accession #: Q9QWJ9
Neuropilin-2
  • Gene Symbol: NRP2
  • Gene ID: 81527
  • Accession #: O35276
Nope
Notch-1
  • Gene Symbol: NOTCH1
  • Gene ID: 25496
  • Accession #: Q07008
Notch-2
  • Gene Symbol: NOTCH2
  • Gene ID: 29492
  • Accession #: Q9QW30
P-Cadherin
Prolactin
RANTES
(CCL5)
SCF
  • Gene Symbol: KITLG
  • Gene ID: 60427
  • Accession #: P21581
TIM-1
(KIM-1)
TIMP-2
TREM-1
TWEAK R
(TNFRSF12)

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Rat Cytokine Array Q4 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Q-Analyzer Bundle Discount

Specify Size
1 x $1,285.73
+

70% off the Q-Analyzer tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).
Total list price $1,846.28
Price for all $1,453.89

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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