Rat IL-1 beta ELISA

RayBio® Rat IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • Q63264
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta)
Target Species:
  • Rat

Assay Format

Detection Range:
80 pg/ml - 50000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
80 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Patyar, Sazal, et al. "Chemopreventive effect of artesunate in 1, 2-dimethylhydrazine-induced rat colon carcinogenesis."?Journal of advanced pharmaceutical technology & research8.3 (2017): 102.
    Species: Rat
    Sample type: plasma
  2. Ali, A. A., et al. "Study on social isolation as a risk factor in development of Alzheimer?s disease in rats."?Brain Disord Ther?6.230 (2017): 2.
    Species: Rat
    Sample type: Tissue
  3. El-Mahdy N., Salem M., El-Sayad M., et al. Bone marrow mononuclear cells enhance anti-inflammatory effects of pravastatin against isoproterenol-induced myocardial infarction in rats. J Immunotoxicol. 2016 May;13(3):393-402. doi: 10.3109/1547691X.2015.1111959.
    Species: Rat
    Sample type: Tissue lysate
  4. Abdelkader NF., Safar MM., Salem H. Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations. Mol Neurobiol. 2014 Dec 15. [Epub ahead of print]. DOI 10.1007/s12035-014-9043-8
    Species: Rat
    Sample type: Tissue Lysate
  5. Allabassi M. The Possible Protective Effects of Zileuton against Pulmonary Fibrosis Induced by Amiodarone in Male Rats. Journal of Natural Sciences Research. Vol.5, No.2, 2015
    Species: Rat
    Sample type: Tissue Lysate
  6. Begum R., Sharma M., Pillai KK., et al. Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation. Pharm Biol. 2015 Mar;53(3):437-45. doi: 10.3109/13880209.2014.923005
    Species: Rat
    Sample type: Serum
  7. Khan A., Jamwai S., Bijjem K., Prakash A., Kumar P. Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3? modulators in 3-nitropropionic acid-induced neurotoxicity in rats. Neuroscience Volume 287, 26 February 2015, Pages 66-77
    Species: Rat
    Sample type: Tissue Lysate
  8. Shah A., Hagawane T., Kshirsagar N., Banerjee R. Surface-active drug loaded lipopolymeric nanohybrid aerosol therapy: potential non-invasive way to mitigate lipopolysaccharide mediated inflammation in murine lungs . RSC Advances 2015, 5, 9683 DOI: 10.1039/c4ra13558j
    Species: Rat
    Sample type: BAL Fluid
  9. Sharma S., Deshmukh R. Vinpocetine attenuates MPTP-induced motor deficit and biochemical abnormalities in Wistar rats. Neuroscience. 2015 Feb 12;286:393-403. doi: 10.1016/j.neuroscience.2014.12.008
    Species: Rat
    Sample type: Tissue Lysate
  10. Tyagi RK., Bisht R., Pant J., et al. Possible role of GABA-B receptor modulation in MPTP induced Parkinson's disease in rats. Exp Toxicol Pathol. 2015 Feb;67(2):211-7. doi: 10.1016/j.etp.2014.12.001.
    Species: Rat
    Sample type: Tissue Lysate
I used rat plasma (heparin anti coagulated) and kidney lysate. After determining an appropriate dilution, using two lanes of the plate, went on to determine the concentrations present in our sample. The assay was easy to use and sensitive, corroborating results from other experiments.

University of Wisconsin
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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