Rat IL-1 beta ELISA

RayBio® Rat IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELR-IL1b-CL-1
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Assay Format

Detection Range:
80 pg/ml - 50000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
80 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q63264
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta)
Target Species:
  • Rat

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Xie, Y., Feng, S.-L., Mai, C.-T., Zheng, Y.-F., Wang, H., Liu, Z.-Q., … Liu, L. (2021). Suppression of up-regulated LXR? by silybin ameliorates experimental rheumatoid arthritis and abnormal lipid metabolism. Phytomedicine, 80, 153339. doi:10.1016/j.phymed.2020.153340
    Species: Rat
    Sample type: Serum
  2. Ashok Priyadarshi, Gaurav K. Keshri, Asheesh Gupta, Hippophae rhamnoides L. leaf extract diminishes oxidative stress, inflammation and ameliorates bioenergetic activation in full-thickness burn wound healing, Phytomedicine Plus, Volume 2, Issue 3, 2022, 100292, ISSN 2667-0313, https://doi.org/10.1016/j.phyplu.2022.100292. (https://www.sciencedirect.com/science/article/pii/S2667031322000756)
    Species: Rat
    Sample type: Tissue Lysate
  3. Li Y, Li S, Li D. Breviscapine Alleviates Cognitive Impairments Induced by Transient Cerebral Ischemia/Reperfusion through Its Anti-Inflammatory and Anti-Oxidant Properties in a Rat Model. ACS Chem Neurosci. 2020 Dec 16;11(24):4489-4498. doi: 10.1021/acschemneuro.0c00697. Epub 2020 Dec 3. PMID: 33270442.
    Species: Rat
    Sample type: Serum
  4. Abdel-Rafei, M. K., & Thabet, N. M. (2020). Modulatory effect of methylsulfonylmethane against BPA/?-radiation induced neurodegenerative alterations in rats: Influence of TREM-2/DAP-12/Syk pathway. Life Sciences, 260, 118410. doi:10.1016/j.lfs.2020.118410 
    Species: Rat
    Sample type: Tissue Lysate
  5. Salama, S. A., & Kabel, A. M. (2020). Taxifolin ameliorates iron overload-induced hepatocellular injury: Modulating PI3K/AKT and p38 MAPK signaling, inflammatory response, and hepatocellular regeneration. Chemico-Biological Interactions, 109230. doi:10.1016/j.cbi.2020.109230 
    Species: Rat
    Sample type: Tissue Lysate
  6. Tian, J., Song, T., Wang, H. et al. Intrathecal Injection of SIRT1-modified Human Mesenchymal Stem Cells Alleviates Neuropathic Pain in Rat. J Mol Neurosci (2020). https://doi.org/10.1007/s12031-020-01717-3
    Species: Rat
    Sample type: Serum
  7. Yassa NW, Khalil S, Saleh SR, Ghareeb DA, El Demellawy MA, El-Sayed MM (2020) Ipriflavone and Ipriflavone loaded albumin nanoparticles reverse lipopolysaccharide induced neuroinflammation in rats. PLoS ONE 15(8): e0237929. https://doi.org/10.1371/journal.pone.0237929
    Species: Rat
    Sample type: Tissue Lysate
  8. Zubrow ME, Margulies SS, Yehya N (2020) Nordihydroguaiaretic acid reduces secondary organ injury in septic rats after cecal ligation and puncture. PLoS ONE 15(8): e0237613. https://doi.org/10.1371/journal.pone.0237613
    Species: Rat
    Sample type: Serum
  9. Ali AA, Al-Attar AS, Refat NAGA, Gomaa M, Eldine ZE, Samy A (2021). Synergistic effect of nanocurcumin and platelet rich plasma on resolving experimentally induced osteoarthritis in albino rats. Adv. Anim. Vet. Sci. 9(1): 26-34.
    Species: Rat
    Sample type: Serum
  10. Zeng Z, Liu Y, Zheng W, Liu L, Yin H, Zhang S, Bai H, Hua L, Wang S, Wang Z, Li X, Xiao J, Yuan Q, Wang Y. MicroRNA-129-5p alleviates nerve injury and inflammatory response of Alzheimer's disease via downregulating SOX6. Cell Cycle. 2019 Nov;18(22):3095-3110. doi: 10.1080/15384101.2019.1669388. Epub 2019 Sep 29. PMID: 31564203; PMCID: PMC6816367.
    Species: Rat
    Sample type: Serum, Tissue Lysate
I used rat serum for the analysis. The assay procedure was simple and reagents quantities were perfect. The assay was easy to use and sensitive, I got the reliable results compare to the from the previous experiments.

Iowa state University
I used rat plasma (heparin anti coagulated) and kidney lysate. After determining an appropriate dilution, using two lanes of the plate, went on to determine the concentrations present in our sample. The assay was easy to use and sensitive, corroborating results from other experiments.

University of Wisconsin
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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