RayBio® Rat IL-10 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P29456
Gene ID:
  • 25325
Gene Symbols:
  • IL-10
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Rat

Assay Format

Detection Range:
10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
10 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. He, Mu Lan, et al. "Interleukin?10 release from astrocytes suppresses neuronal apoptosis via the TLR 2/NF ?B pathway in a neonatal rat model of hypoxic?ischemic brain damage."?Journal of neurochemistry?142.6 (2017): 920-933.
    Species: Rat
    Sample type: Tissue/ lysates
  2. Ram M., Singh V., Kumawat S., et al. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int Immunopharmacol. 2016 Jan;30:137-49. doi: 10.1016/j.intimp.2015.11.037
    Species: Rat
    Sample type: Tissue lysate
  3. Abdelkader NF., Safar MM., Salem H. Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations. Mol Neurobiol. 2014 Dec 15. [Epub ahead of print]. DOI 10.1007/s12035-014-9043-8
    Species: Rat
    Sample type: Tissue Lysate
  4. Vashistha B., Sharma A., Jain V. Ameliorative potential of ferulic acid in vincristine-induced painful neuropathy in rats: An evidence of behavioral and biochemical examination. Nutr Neurosci. 2014 Dec 15. [Epub ahead of print] http://dx.doi.org/10.1179/1476830514Y.0000000165
    Species: Rat
    Sample type: Tissue Lysate
  5. Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718
    Species: Rat
    Sample type: Tissue Lysate
  6. Wang X., Zhou J., Han Z., Tang F. Protective effects of Semen Crotonis Pulveratum on trinitrobenzene sulphonic acid-induced colitis in rats and H?O?-induced intestinal cell apoptosis in vitro. Int J Mol Med. 2015 Jun;35(6):1699-707. doi: 10.3892/ijmm.2015.2175.
    Species: Rats
    Sample type: Serum
  7. Ram M., Singh V., Kumawat S., et al. Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats. Eur J Pharmacol. 2015 Oct 5;764:9-21. doi: 10.1016/j.ejphar.2015.06.029.
    Species: Rat
    Sample type: Tissue Lysate
  8. El-Gowilly S., Helmy M., El-Gowelli H. Pioglitazone ameliorates methotrexate-induced renal endothelial dysfunction via amending detrimental changes in some antioxidant parameters, systemic cytokines and Fas production. Vascul Pharmacol. 2015 Nov;74:139-50. doi: 10.1016/j.vph.2015.07.002.
    Species: Rat
    Sample type: Serum
  9. Eid F., Mohamed A., Aly A., Ibrahim N. Effects of swine flu (H1N1) vaccine on albino rats. Journal of Bioscience and Applied Research ,2015 Vol.1, No.3, PP.113-126 pISSN: 2356-9174, eISSN: 2356-9182
    Species: Rat
    Sample type: Conditioned Media
  10. Gu Y., Zhang Y., Bi Y., et al. Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NF?B pathway in rats with hypoxic-ischemic brain damage. Mol Brain. 2015 Oct 17;8(1):65. doi: 10.1186/s13041-015-0157-3.
    Species: Rat
    Sample type: Conditioned Media

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Not for diagnostic or therapeutic use.

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