RayBio® Rat IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P17108
Gene ID:
  • 116562
Gene Symbols:
  • IL-2
  • IL2
Protein Name & Synonyms:
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Target Species:
  • Rat

Assay Format

Detection Range:
0.1 ng/ml - 30 ng/ml
Recommended Dilution (serum & plasma):
2 fold
100 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Choudhary A., Devi R. Longer period of oral administration of aspartame on cytokine response in Wistar albino rats. Endocrinol Nutr. 2015 Mar;62(3):114-22. doi: 10.1016/j.endonu.2014.11.004
    Species: Rat
    Sample type: Serum
  2. Mohanty S., Sawmy M., Middah S., et al. Analgesic, Anti- inflammatory, Anti- lipoxygenase Activity and Characterization of Three Bioactive Compounds in the Most Active Fraction of Leptadenia reticulata (Retz.)Wight & Arn. - A Valuable Medicinal Plant. Iran J Pharm Res. 2015 Summer;14(3):933-42.
    Species: Rat
    Sample type: Serum
  3. Oyewo EB, Adewale A, Abdulfatai Ayoade A, Akanji MA. Repeated Oral Administration of Aqueous Leaf Extract of Moringa oleifera modulated immunoactivities in Wistar Rats. J Nat Sci Res. 2013;3(6):100-109.
    Species: Rat
    Sample type: Serum
  4. J Tuorkey, Muobarak, Karolin K Abdul-Aziz, and Abdel-Aziz A Zidan. "Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats."?Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders)?13.3 (2013): 269-274.
    Species: Rat
    Sample type: Serum
  5. Afolabi, Olusegun Kayode, et al. "Oxidative indices correlate with dyslipidemia and pro-inflammatory cytokine levels in fluoride-exposed rats."?Archives of Industrial Hygiene and Toxicology?64.4 (2013): 521-529.
    Species: Rat
    Sample type: Plasma
  6. Oyewo EB, Adetutu A, Adebisi JA.Immunomodulatory activities of Yoyo bitters: recommended dose precipitated inflammatory responses in male Wistar rats. Pak J Biol Sci. 2013 Dec 15;16(24):1904-12..
    Species: Rat
    Sample type: Serum
  7. Oyevvo, Emmanuel Bukoye. "Sub-chronic Administration of Febi Super Bitters Triggered In?ammatory Responses in Male Wistar Rats."?J. Med. Sci?13.8 (2013): 692-699.
    Species: Rat
    Sample type: Serum
  8. Afolabi, O.K., Oyewo, E.B., Adekunle, A.S., Adedosu, O.T. & Adedeji, A.L. (2013). Oxidative Indices Correlate with Dyslipidemia and Pro-Inflammatory Cytokine Levels in Fluoride-Exposed Rats.?Arhiv za higijenu rada i toksikologiju, 64?(4), 521-528. https://doi.org/10.2478/10004-1254-64-2013-2351
    Species: Rat
    Sample type: Plasma
  9. Afolabi, O., et al. "Assessment Of Lipid Peroxidation Markers And Roinflammatory Cytokines In Arsenite-Exposed Rats."?Assessment?2 (2013): 8-18.
    Species: Rat
    Sample type: Plasma
  10. Adekunle A.S., Afolabi, O.K., Oyewo, B.E: Therapeutic efficacy of Tapinanthus globiferus on acetaminophen induced nephrotoxicity, inflammatory reactions and oxidative stress in albino rats. Report and Opinion, 2012;4(3):1-5
    Species: Rat
    Sample type: Serum

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