RayBio® Rat IL-4 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P20096
Gene ID:
  • 287287
Gene Symbols:
  • IL-4
  • IL4
Protein Name & Synonyms:
Interleukin-4 (IL-4) (B-cell IGG differentiation factor) (B-cell growth factor 1) (B-cell stimulatory factor 1) (BSF-1) (Lymphocyte stimulatory factor 1)
Target Species:
  • Rat

Assay Format

Detection Range:
1.5 pg/ml - 160 pg/ml
Recommended Dilution (serum & plasma):
2 fold
1.5 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Miltonprabu S., Manoharan N. Hepatoprotective effect of grape seed proanthocyanidins on Cadmium-induced hepatic injury in rats: Possible involvement of mitochondrial dysfunction, inflammation and apoptosis. Toxicology Reports Volume 3, 2016, Pages 63-77
    Species: Rat
    Sample type: Tissue lysate
  2. Choudhary A., Devi R. Longer period of oral administration of aspartame on cytokine response in Wistar albino rats. Endocrinol Nutr. 2015 Mar;62(3):114-22. doi: 10.1016/j.endonu.2014.11.004
    Species: Rat
    Sample type: Serum
  3. Francis A., Ganapathy S., Palla V., et al. One time nose-only inhalation of MWCNTs: Exploring themechanism of toxicity by intermittent sacrifice in Wistar rats. Toxicology Reports 2 (2015) 111-120. http://dx.doi.org/10.1016/j.toxrep.2015.02.003
    Species: Rat
    Sample type: BAL Fluid
  4. Lin Y., Zhang L., Dai Y., et al. Expression of interleukin-9 and its upstream stimulating factors in rats with ischemic stroke. Neurol Sci. 2015 Jun;36(6):913-20. doi: 10.1007/s10072-015-2096-2.
    Species: Rat
    Sample type: Tissue Lysate
  5. Zhang Y., Zhang S., Tsui N. Mesenteric Lymph Duct Drainage Attenuates Acute Lung Injury in Rats with Severe Intraperitoneal Infection. Inflammation, Vol. 38, No. 3, June 2015, p1239 - 1249. DOI: 10.1007/s10753-014-0091-z
    Species: Rat
    Sample type: Tissue Lysate
  6. Ohtani S., Usuiyama A., Maeda M., et al. The effects of radio-frequency electromagnetic fields on T cell function during development. J Radiat Res. 2015 May;56(3):467-74. doi: 10.1093/jrr/rru126
    Species: Rat
    Sample type: Serum
  7. Arafa M., Mohammad N., Atteia H. Fenugreek seed powder mitigates cadmium-induced testicular damage and hepatotoxicity in male rats. Experimental and Toxicologic Pathology. Volume 66, Issues 5-6, Pages 219-276
    Species: Rat
    Sample type: Tissue Lysate
  8. Koshy S., Huq R., Tanner M., et al. Blocking KV1.3 channels inhibits Th2 lymphocyte function and treats a rat model of asthma. J Biol Chem. 2014 May 2;289(18):12623-32. doi: 10.1074/jbc.M113.517037
    Species: Rat
    Sample type: BAL Fluid
  9. Amdekar, Sarika, et al. "Lactobacillus casei and Lactobacillus acidophilus regulate inflammatory pathway and improve antioxidant status in collagen-induced arthritic rats."?Journal of Interferon & Cytokine Research?33.1 (2013): 1-8.
    Species: Rat
    Sample type: Serum
  10. Kandhare, Amit D., et al. "Elucidation of molecular mechanism involved in neuroprotective effect of Coenzyme Q10 in alcohol?induced neuropathic pain."?Fundamental & clinical pharmacology?27.6 (2013): 603-622.
    Species: Rat
    Sample type: Tissue Lysate

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