Rat IL-6 ELISA

RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$420.00  
ELR-IL6-CL-1
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Assay Format

Detection Range:
30 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
30 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P20607
Gene ID:
  • 24498
Gene Symbols:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6)
Target Species:
  • Rat

Target Background

Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Chaudhary S, Sahu U, Parvez S. Melatonin attenuates branch chain fatty acid induced apoptosis mediated neurodegeneration. Environ Toxicol. 2021 Apr;36(4):491-505. doi: 10.1002/tox.23055. Epub 2020 Nov 21. PMID: 33219756.
    Species: Rat
    Sample type: Tissue Lysate
  2. Ashok Priyadarshi, Gaurav K. Keshri, Asheesh Gupta, Hippophae rhamnoides L. leaf extract diminishes oxidative stress, inflammation and ameliorates bioenergetic activation in full-thickness burn wound healing, Phytomedicine Plus, Volume 2, Issue 3, 2022, 100292, ISSN 2667-0313, https://doi.org/10.1016/j.phyplu.2022.100292. (https://www.sciencedirect.com/science/article/pii/S2667031322000756)
    Species: Rat
    Sample type: Tissue Lysate
  3. Li Y, Li S, Li D. Breviscapine Alleviates Cognitive Impairments Induced by Transient Cerebral Ischemia/Reperfusion through Its Anti-Inflammatory and Anti-Oxidant Properties in a Rat Model. ACS Chem Neurosci. 2020 Dec 16;11(24):4489-4498. doi: 10.1021/acschemneuro.0c00697. Epub 2020 Dec 3. PMID: 33270442.
    Species: Rat
    Sample type: Serum
  4. Du, Z., Wang, J., Lu, Y., Ma, X., Wen, R., Lin, J., … Jiang, Y. (2020). The cardiac protection of Baoyuan decoction via gut-heart axis metabolic pathway. Phytomedicine, 153322. doi:10.1016/j.phymed.2020.153322 
    Species: Rat
    Sample type: Plasma
  5. Garg S, Khan SI, Malhotra RK, Sharma MK, Kumar M, Kaur P, Nag TC, RumaRay, Bhatia J, Arya DS. The molecular mechanism involved in cardioprotection by the dietary flavonoid fisetin as an agonist of PPAR-? in a murine model of myocardial infarction. Arch Biochem Biophys. 2020 Nov 15;694:108572. doi: 10.1016/j.abb.2020.108572. Epub 2020 Sep 12. PMID: 32926843.
    Species: Rat
    Sample type: Serum
  6. Salama, S. A., & Kabel, A. M. (2020). Taxifolin ameliorates iron overload-induced hepatocellular injury: Modulating PI3K/AKT and p38 MAPK signaling, inflammatory response, and hepatocellular regeneration. Chemico-Biological Interactions, 109230. doi:10.1016/j.cbi.2020.109230 
    Species: Rat
    Sample type: Tissue Lysate
  7. Abo El-Nasr NME, Saleh DO, Mahmoud SS, Nofal SM, Abdelsalam RM, Safar MM, El-Abhar HS. Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways. Eur J Pharmacol. 2020 May 5;874:173010. doi: 10.1016/j.ejphar.2020.173010. Epub 2020 Feb 14. PMID: 32067934.
    Species: Rat
    Sample type: Tissue Lysate
  8. Tian, J., Song, T., Wang, H. et al. Intrathecal Injection of SIRT1-modified Human Mesenchymal Stem Cells Alleviates Neuropathic Pain in Rat. J Mol Neurosci (2020). https://doi.org/10.1007/s12031-020-01717-2
    Species: Rat
    Sample type: Serum
  9. Ali AA, Al-Attar AS, Refat NAGA, Gomaa M, Eldine ZE, Samy A (2021). Synergistic effect of nanocurcumin and platelet rich plasma on resolving experimentally induced osteoarthritis in albino rats. Adv. Anim. Vet. Sci. 9(1): 26-34.
    Species: Rat
    Sample type: Serum
  10. Mora S, Martín-González E, Prados-Pardo Á, Moreno J, López MJ, Pilar-Cuellar F, Castro E, Díaz Á, Flores P, Moreno M. Increased vulnerability to impulsive behavior after streptococcal antigen exposure and antibiotic treatment in rats. Brain Behav Immun. 2020 Oct;89:675-688. doi: 10.1016/j.bbi.2020.08.010. Epub 2020 Aug 13. PMID: 32798664.
    Species: Rat
    Sample type: Tissue Lysate
The kit is wonderful, I used Kidney homogenates.

University of Houston
Excellent results; easy to use.

SUNY Downstate Medical Center
Work really well on rat adipose tissue

WVU
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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