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Assay Format

Number of Targets Detected:

Species Detected:

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Andpro | Ccl11 | Ccl2 | Ccl20 | Ccl27 | Ccl3 | Ccl5 | CSF2 | Cxcl1 | Cxcl2 View more

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A Gene Symbol: Inhba Gene ID: 29200 Accession #: P18331 ELISA Kit: RayBio Rat Activin-A ELISA	MIP-3 alpha (CCL20) Gene Symbol: Ccl20 Scya20 St38 Gene ID: 29538 Accession #: P97884 ELISA Kit: RayBio Rat MIP-3 alpha/CCL20 ELISA	CINC-1 (CXCL1) Gene Symbol: Cxcl1 Cinc1 Gro Scyb1 Gene ID: 81503 Accession #: P14095 ELISA Kit: RayBio Rat CINC-1 ELISA	CINC-2 Gene Symbol: Cxcl3 Cinc2 Gene ID: 171551 Accession #: Q10746 ELISA Kit: RayBio Rat CINC-2 ELISA	CINC-3 Gene Symbol: Cxcl2 Cinc3 Mip-2 Mip2 Scyb2 Gene ID: 114105 Accession #: P30348 ELISA Kit: RayBio Rat CINC-3 ELISA
CRP (C-Reactive Protein) Gene Symbol: Andpro Crp1 Gene ID: 25030 Accession #: P22282 ELISA Kit: RayBio Rat CRP ELISA	CTACK (CCL27) Gene Symbol: Ccl27 Ccl27_predicted rCG_54915 Gene ID: 362505 Accession #: D4AAL6 ELISA Kit: RayBio Rat CTACK ELISA	Eotaxin-1 (CCL11) Gene Symbol: Ccl11 Scya11 Gene ID: 29397 Accession #: P97545 ELISA Kit: RayBio Rat Eotaxin-1 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Apt1 Tnfrsf6 Gene ID: 246097 Accession #: Q63199 ELISA Kit: RayBio Rat Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: Faslg Apt1Lg1 Cd95l Fasl Tnfsf6 Gene ID: 25385 Accession #: P36940 ELISA Kit: RayBio Rat FasL ELISA
Galectin-1 Gene Symbol: Lgals1 Gene ID: 56646 Accession #: P11762 ELISA Kit: RayBio Rat Galectin-1 ELISA	Galectin-3 Gene Symbol: Lgals3 Gene ID: 83781 Accession #: P08699 ELISA Kit: RayBio Rat Galectin-3 ELISA	GM-CSF Gene Symbol: Csf2 Csfgm Gene ID: 116630 Accession #: P48750 ELISA Kit: RayBio Rat GM-CSF ELISA	gp130 (sgp130) Gene Symbol: Il6st Accession #: P40190 ELISA Kit: RayBio Rat gp130 ELISA	ICAM-1 (CD54) Gene Symbol: Icam1 Icam-1 Gene ID: 25464 Accession #: Q00238 ELISA Kit: RayBio Rat ICAM-1 ELISA
IFN-gamma Gene Symbol: Ifng Gene ID: 25712 Accession #: P01581 ELISA Kit: RayBio Rat IFN-gamma ELISA	IL-10 Gene Symbol: Il10 Il-10 Gene ID: 25325 Accession #: P29456 ELISA Kit: RayBio Rat IL-10 ELISA	IL-13 Gene Symbol: Il13 Il-13 Gene ID: 116553 Accession #: P42203 ELISA Kit: RayBio Rat IL-13 ELISA (For Lysates)	IL-17F Gene Symbol: Il17f Gene ID: 301291 Accession #: Q5BJ95 ELISA Kit: RayBio Rat IL-17F ELISA	IL-18 Gene Symbol: Il18 Igif Gene ID: 29197 Accession #: P97636 ELISA Kit: RayBio Rat IL-18 ELISA
IL-1 alpha (IL-1 F1) Gene Symbol: Il1a Gene ID: 24493 Accession #: P16598 ELISA Kit: RayBio Rat IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: Il1b Accession #: Q63264 ELISA Kit: RayBio Rat IL-1 beta ELISA	IL-2 Gene Symbol: Il2 Il-2 Gene ID: 116562 Accession #: P17108 ELISA Kit: RayBio Rat IL-2 ELISA	IL-22 Gene Symbol: Il22 RGD1561292_predicted rCG_48772 Gene ID: 500836 Accession #: G3V6X6 ELISA Kit: RayBio Rat IL-22 ELISA	IL-3 Gene Symbol: Il3 Il-3 Accession #: P04823 ELISA Kit: RayBio Rat IL-3 ELISA
IL-4 Gene Symbol: Il4 Il-4 Gene ID: 287287 Accession #: P20096 ELISA Kit: RayBio Rat IL-4 ELISA	IL-6 Gene Symbol: Il6 Il-6 Gene ID: 24498 Accession #: P20607 ELISA Kit: RayBio Rat IL-6 ELISA	IL-7 Gene Symbol: Il7 Accession #: P56478 ELISA Kit: RayBio Rat IL-7 ELISA	LIX Gene Symbol: Cxcl5 Scyb5 Gene ID: 60665 Accession #: P97885 ELISA Kit: RayBio Rat LIX ELISA	MCP-1 (CCL2) Gene Symbol: Ccl2 Je Mcp1 Scya2 Gene ID: 24770 Accession #: P14844 ELISA Kit: RayBio Rat MCP-1 ELISA
MIG (CXCL9) Gene Symbol: Cxcl9 Mig rCG_60435 Gene ID: 246759 Accession #: Q8K4B1 ELISA Kit: RayBio Rat MIG/CXCL9 ELISA	MIP-1 alpha (CCL3) Gene Symbol: Ccl3 Mip1a Scya3 Gene ID: 25542 Accession #: P50229 ELISA Kit: RayBio Rat MIP-1alpha ELISA	PDGF-BB ELISA Kit: RayBio Rat PDGF-BB ELISA	RANTES (CCL5) Gene Symbol: Ccl5 Scya5 Accession #: P50231 ELISA Kit: RayBio Rat RANTES/CCL5 ELISA	TIMP-2 Gene Symbol: Timp2 Timp-2 Gene ID: 29543 Accession #: P30121 ELISA Kit: RayBio Rat TIMP-2 ELISA
TNF alpha Gene Symbol: Tnf Tnfa Tnfsf2 Gene ID: 103694380 24835 Accession #: P16599 ELISA Kit: RayBio Rat TNF-alpha ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Rat Inflammation Antibody Array C3 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

I have used the AAR-INF-3 array and have observed consistent results in determining cytokines in the kidney sample of male obese Zucker rats. Experimental conditions were similar to the manufacturer's instructions.

Sanket Patel
University of Houston College of Pharmacy 06/25/2022, 05:02 PM

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