Rat Inflammation Array C3

RayBio® C-Series Rat Inflammation Antibody Array 3 Kit. Detects 36 Rat Inflammation Factors. Suitable for all liquid sample types.

$804.00  
AAR-INF-3-8
+

Assay Format

Number of Targets Detected:
36
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
Andpro | Ccl11 | Ccl2 | Ccl20 | Ccl27 | Ccl3 | Ccl5 | CSF2 | Cxcl1 | Cxcl2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
MIP-3 alpha
(CCL20)
CINC-1
(CXCL1)
CINC-2
CINC-3
CRP
(C-Reactive Protein)
CTACK
(CCL27)
Eotaxin-1
(CCL11)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Galectin-1
Galectin-3
GM-CSF
gp130
(sgp130)
ICAM-1
(CD54)
IFN-gamma
IL-10
IL-13
IL-17F
IL-18
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-22
IL-3
IL-4
IL-6
IL-7
LIX
MCP-1
(CCL2)
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
PDGF-BB
RANTES
(CCL5)
TIMP-2
TNF alpha
       

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Rat Inflammation Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
I have used the AAR-INF-3 array and have observed consistent results in determining cytokines in the kidney sample of male obese Zucker rats. Experimental conditions were similar to the manufacturer's instructions.

University of Houston College of Pharmacy
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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