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Rat Inflammation Array Q1

Quantibody® Rat Inflammation Array 1 Kit. Detects 10 Rat Inflammatory Factors. Suitable for all liquid sample types.

Old price: $428.24
$299.77  
You save: $128.47 (30%)
QAR-INF-1-1
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Antigen Information

Gene Symbols:
  • CCL2
  • IFNG
  • IL10
  • IL13
  • IL1A
  • IL1B
  • IL2
  • IL4
  • IL6
  • TNF

Assay Format

Pathway:
  • JAK/STAT Signaling
  • MAPK Signaling
  • NFkB Signaling
Number of Targets Detected:
10
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-4
IL-6
IL-10
IL-13
MCP-1
(CCL2)
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Rat Inflammation Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Bigelman E., Cohen L., Aharon-Hananel G., et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLoS One. 2018 Jun 11;13(6):e0198196. doi: 10.1371/journal.pone.0198196
    Species: Rat
    Sample type: Plasma
  2. Zheng S., Pan Y., Wang C., et al. HMGB1 Turns Renal Tubular Epithelial Cells into Inflammatory Promoters by Interacting with TLR4 During Sepsis. J Interferon Cytokine Res. 2016 Jan;36(1):9-19. doi: 10.1089/jir.2015.0067.
    Species: Rat
    Sample type: Conditioned Media
  3. Chang Y., Jia X., Sun X., et al. APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis. Mol Immunol. 2015 Mar;64(1):90-8. doi: 10.1016/j.molimm.2014.11.002.
    Species: Rat
    Sample type: Conditioned Media
  4. Jiang S., Zhang M., Tian Z., et al. The monoacylglycerol lipase inhibitor JZL184 decreases inflammatory response in skeletal muscle contusion in rats. Eur J Pharmacol. 2015 Aug 15;761:1-10. doi: 10.1016/j.ejphar.2015.04.018.
    Species: Rat
    Sample type: Tissue Lysate
  5. Zhang M., Jiang S., Tian Z., et al. CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3491-502. eCollection 2015
    Species: Rat
    Sample type: Tissue Lysate
  6. Willett N., et al. Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development. Arthritis Res Ther. 2014;16: R47.
    Species: Rat
    Sample type: Synovial Fluid
Easy to use and works great.

Cedars-Sinai Medical Center
Great kit and easy to use

UNMC
Good

unmc
This array was very easy to use and provided great results for my project. The only downside was I did not have the appropriate scanner but the company offers complimentary scanning.

University of Florida
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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